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lipopolysaccharides induced apoptosis human lung fibroblasts  (ATCC)


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    ATCC lipopolysaccharides induced apoptosis human lung fibroblasts
    Figure 1. Vanillic acid suppressed cell <t>apoptosis</t> of lipopolysaccharides-treated human lung <t>fibroblasts.</t> (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.
    Lipopolysaccharides Induced Apoptosis Human Lung Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/apoptosis+human+lung+fibroblasts/WI-38/10__15586_slash_qas__v14i1__1018-47-11-22
    Average 99 stars, based on 3106 article reviews
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    1) Product Images from "Vanillic acid alleviates lipopolysaccharides-induced endoplasmic reticulum stress and inflammation in human lung fibroblasts by `inhibiting MAPK and NF-κB pathways"

    Article Title: Vanillic acid alleviates lipopolysaccharides-induced endoplasmic reticulum stress and inflammation in human lung fibroblasts by `inhibiting MAPK and NF-κB pathways

    Journal: Quality Assurance and Safety of Crops & Foods

    doi: 10.15586/qas.v14i1.1018

    Figure 1. Vanillic acid suppressed cell apoptosis of lipopolysaccharides-treated human lung fibroblasts. (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.
    Figure Legend Snippet: Figure 1. Vanillic acid suppressed cell apoptosis of lipopolysaccharides-treated human lung fibroblasts. (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.

    Techniques Used: Incubation, Expressing

    Related Articles

    Cell Culture:

    Article Title: Vanillic acid alleviates lipopolysaccharides-induced endoplasmic reticulum stress and inflammation in human lung fibroblasts by `inhibiting MAPK and NF-κB pathways
    Article Snippet: .. Materials and methods Effect of vanillic acid on cell viability and lipopolysaccharides-induced apoptosis Human lung fibroblasts (WI-38 and MRC-5) were acquired from American Type Culture Collection (Manassas, VA, USA), and cultured in Dulbecco's modified Eagle’s medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum and penicillin–streptomycin (Invitrogen). .. Cells were incubated with 1-, 2-, 5-, or 10-μg/mL lipopolysaccharides (Sigma-Aldrich, St Louis, MO, USA) for 12 h to induce inflammation.

    Modification:

    Article Title: Vanillic acid alleviates lipopolysaccharides-induced endoplasmic reticulum stress and inflammation in human lung fibroblasts by `inhibiting MAPK and NF-κB pathways
    Article Snippet: .. Materials and methods Effect of vanillic acid on cell viability and lipopolysaccharides-induced apoptosis Human lung fibroblasts (WI-38 and MRC-5) were acquired from American Type Culture Collection (Manassas, VA, USA), and cultured in Dulbecco's modified Eagle’s medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum and penicillin–streptomycin (Invitrogen). .. Cells were incubated with 1-, 2-, 5-, or 10-μg/mL lipopolysaccharides (Sigma-Aldrich, St Louis, MO, USA) for 12 h to induce inflammation.



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    ATCC lipopolysaccharides induced apoptosis human lung fibroblasts
    Figure 1. Vanillic acid suppressed cell <t>apoptosis</t> of lipopolysaccharides-treated human lung <t>fibroblasts.</t> (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.
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    ATCC apoptosis human lung fibroblasts
    Figure 1. Vanillic acid suppressed cell <t>apoptosis</t> of lipopolysaccharides-treated human lung <t>fibroblasts.</t> (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.
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    ATCC apoptosis mouse nb 2a cells
    Figure 1. Vanillic acid suppressed cell <t>apoptosis</t> of lipopolysaccharides-treated human lung <t>fibroblasts.</t> (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.
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    Figure 1. Vanillic acid suppressed cell apoptosis of lipopolysaccharides-treated human lung fibroblasts. (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.

    Journal: Quality Assurance and Safety of Crops & Foods

    Article Title: Vanillic acid alleviates lipopolysaccharides-induced endoplasmic reticulum stress and inflammation in human lung fibroblasts by `inhibiting MAPK and NF-κB pathways

    doi: 10.15586/qas.v14i1.1018

    Figure Lengend Snippet: Figure 1. Vanillic acid suppressed cell apoptosis of lipopolysaccharides-treated human lung fibroblasts. (A) Incubation with lipopolysaccharides (more than 2 μg/mL) reduced cell viabilities of WI-38 and MRC-5. (B) Vanillic acid (40 μg/mL) reduced cell viabilities of WI-38 and MRC-5, while vanillic acid below 20 μg/mL demonstrated no significant effect on the cell viabilities of WI-38 and MRC-5. (C) Vanillic acid enhanced cell viabilities of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage- dependent manner. (D) Vanillic acid treatment suppressed cell apoptosis of lipopolysaccharides-treated WI-38 and MRC-5 in a dosage-dependent manner. (E) Vanillic acid treatment decreased protein expression of BAX while increasing BCL2 and cleaved caspase 3 in lipopolysaccharides-treated WI-38 and MRC-5. *P < 0.05, **P < 0.01, ***P < 0.001.

    Article Snippet: Materials and methods Effect of vanillic acid on cell viability and lipopolysaccharides-induced apoptosis Human lung fibroblasts (WI-38 and MRC-5) were acquired from American Type Culture Collection (Manassas, VA, USA), and cultured in Dulbecco's modified Eagle’s medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum and penicillin–streptomycin (Invitrogen).

    Techniques: Incubation, Expressing